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Zetasizer Pro


Malvern Panalytical

Malvern Panalytical

Zetasizer Pro Enhanced speed and ease of use The new Zetasizer Pro is a robust and versatile system for the measurement of particle and molecular size and electrophoretic mobility and zeta potential of nanoparticles. It enables size measurements to be performed up to three times quicker, compared to previous models, accelerating sample throughput and freeing up operator and system time for other tasks

Key Differences

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Features & Benefits

Even novice users will produce the highest-quality measurements, thanks to the intuitive interface and the deep learning empowered data quality advice system that highlights issues and provides advice on how to overcome them.  

  • Dynamic Light Scattering (DLS) measures particle and molecule size, from below 1 nm to 10 µm

  • Electrophoretic Light Scattering (ELS) measures the zeta potential of particles and molecules, indicating sample stability and/or propensity to aggregate

  • Non-Invasive Back-Scatter (NIBS) optics for versatile, high sensitivity, particle and molecular size measurements

  • M3-PALS with Constant Current Mode for zeta potential and electrophoretic mobility measurements

  • ZS Xplorer software suite for simple and flexible method set-up and data analysis

  • Adaptive Correlation for faster, more reproducible sizing measurements with less sample preparation

  • Deep Learning empowered data quality system for assessment of any size data quality issues, with clear advice on how to improve results

  • Optical filter wheel with fluorescence filter to allow measurement of fluorescent samples without impairing overall system sensitivity.  Also includes polarization filters for DDLS measurements

  • The optional MPT-3 Auto titrator enables study of the effect of changes in pH

  • A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume and concentration

The Malvern Panalytical Zetasizer Pro can be used in many industry applications, including:


The Zetasizer is an essential analytical tool for many academic laboratories worldwide, finding use in all applications where particle or molecular size and zeta potential analysis is required. The breadth of applications covered by Zetasizer systems, coupled with tens of thousands of scientific citations, have earned these instruments a central space in many scientific faculties. 


Variations in molecule concentration, external temperature, level of agitation and time all impact the stability of biological molecules. Instabilities mainly result in denaturation and aggregation of the molecule through which they can lose their biological function , elicit an immune response and consequently limit its therapeutic viability. Factors affecting therapeutic efficacy and immunogenicity therefore need to be monitored throughout the development pipeline especially at early stages to de-risk the biological drug molecule and limit late-stage failures. The Zetasizer range provides high quality, stability indicating data for biological drug molecules in a short time frame, enabling this measurement to be implemented throughout the development pipeline.   

Food and drink 

Zetasizer systems are used to analyze particle size and zeta potential to improve the appearance and taste of foods, drinks and flavorings, and optimize dispersion and emulsion stability to extend product shelf life and improve product performance.


Zetasizer measurements of nanoparticle size distribution, dispersion properties, stability and propensity to aggregate are crucial to the design of new nanomaterials. The very large surface area of such materials may result in novel physical and chemical properties, such as increased catalytic activity, improved solubility or unexpected optical or toxicological behaviour.

Paints, inks and coatings

Paint, ink and coating formulations must be stable to keep them in an optimum state over time without change or aggregation. Particle size and zeta potential, measured by the Zetasizer instruments, play important roles in determining a product’s properties, such as dispersibility, color, strength, finish, durability and shelf life.


The correct particle size and zeta potential can help ensure the production of effective therapeutics, delivered safely. Zetasizer systems are used to characterize the stability and quality of dispersions, emulsions and creams, reducing formulation time and speeding new products to market.

Technical Specification

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Particle size and molecular size

  • Measurement principle: Dynamic light scattering (Non- Invasive Back-Scatter - NIBS)

  • Dynamic light scattering (13°, 173°)

  • Measurement angle: 173°, 13° ¹

  • Measurement range: Diameter: 0.3 nm - 10 μm ²

  • Minimum sample volume: 12 μL

  • Concentration range:Minimum sample concentration ³:  

  • NIBS (173°): 0.1 mg/mL 

  • Forward angle (13°): 10 mg/mL 

  • Side angle (90°): N/A

  • MADLS: N/A

  • Maximum sample concentration ⁴ ⁵:  40% w/v 


Zeta potential

  • Measurement principle:Mixed-mode measurement, phase analysis light scattering (M3-PALS)

  • Size range:3.8 nm – 100 μm (diameter) ²

  • Minimum sample volume:20 μL ⁶

  • Concentration range: 10 mg/mL ³ - 40% w/v ⁴ ⁵

  • Sample conductivity range: Maximum: 260 mS/cm 

  • Accuracy: ± 10%



  • Detector: Avalanche photodiode

  • Temperature control range: 0°C - 120°C ⁷

  • Dimensions (W, D, H): 322 mm x 565 mm x 245 mm

  • Weight: 19 kg

  • Power: AC 100-240 V, 50-60 Hz, 4.0 A

  • Power consumption: Max. 100 W, 45 W typical

  • Ambient conditions: +10°C to +35°C (+50°F to +95°F) 35% - 80% RH (non-condensing)

  • Interface: USB 2.0 or higher


1. Water as sample dispersant; 

2. Peak mode range (diameter) 0.6 nm - 10 μm; sample & sample preparation dependent; 

3. Measured using 15 kDa protein; 

4. Sample-dependent; 

5. Measured using bile acid; 

6. Using diffusion barrier method; 

7. Temperature accuracy 0.1°C at 25°C, 0.2°C at 0°C, 0.5°C at 90°C, 2.5°C at 120°C

Zetasizer Pro


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