
OMNISEC
Description
Malvern Panalytical
OMNISEC The world’s most advanced multi-detector GPC/SEC system Malvern Panalytical's OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.
Key Differences
< click here
OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.
As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.
Market leading high sensitive light scattering
-
Measure low molecular weight samples with greater accuracy (down to 200 g/mol)
-
Measure lower sample concentrations (newly synthesized polymers and proteins)
-
Measure low dn/dc samples with greater accuracy and sensitivity
High sensitivity Refractive index
-
Greater accuracy and less subjectivity at lower concentrations
-
Measure lower concentraions of highly viscous materials to prevent column overload
Integrated robust differential viscometer
-
Measure Iv of samples to determine structure and branching
-
Robust to all GPC solvents and a wide pH range broadening available applications
Mechanical viscometer bridge autobalance
-
Improved viscometer data quality and accuracy
Fully integrated detector module
-
Better temperature control and reduced interdetecor volume = better chromatography
-
Improved separation quality and resolution
All detectors in series
-
no detectors in a parallel flow path meaning higher sensitivity and better quality results
Work flow orientated software
-
Makes GPC/SEC measurements and analysis easy and intuitive
How it works
In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:
Degasser
-
Rapid mobile-phase exchange thanks to low volume degasser
Pump
-
Rapid mobile-phase exchange thanks to self-priming pump
-
Reduced service requirement thanks to novel continuous back seal flushing mechanism
Autosampler
-
Protect samples from aggregation with autosampler temperature control
-
Use your preferred sample format; vials or 96-well microtiter plates
-
Reduce sample use with zero-waste mode
Column Oven
-
Temperature control of up to 6 analytical columns
Refractive Index
-
Measure the concentration of any solute with excellent sensitivity
UV/Vis PDA
-
Measure absorbance at any wavelength from 190-900 nm
-
Assess changes in absorbance spectra
-
Characterize copolymers
Light Scattering
-
Measure absolute molecular weight in a wider range of applications
-
Measure as little as 100 ng of material
-
Measure molecular weights down to 200 Da
Viscometer
-
Better intrinsic viscosity measurements thanks to automated balancing
-
Reduced service cost and time thanks to user-exchangeable capillaries
Technical Specification
< click here
OMNISEC RESOLVE
Pump flow rate: 0.005 - 10 mL/min
Flow rate accuracy: ±1%
Pressure range: 0 - 5000 PSI (34.5 MPa)
Pulsation: 0.15% @ 1 mL/min in water
Dimensions (W, D, H): 420, 640, 890 mm
Weight: 62 kg
Software: OMNISEC software v10 (or later)
Data acquisition rate: 100 Hz
AUTOSAMPLER
Capacity: Up to 192
Sample container types: HPLC vials or 96-well microtiter plates
Injection volume: 1 - 300 µL
Injection volume accuracy: >99.5%
Injection volume precision:
-
<0.3% RSD in full loop mode
-
<0.5% RSD in partial loop mode
-
<1% RSD in µL pickup mode
Injection overhead volume: 0 µL in µL pickup mode
Syringe volume: 250 µL standard
Temperature control range: 4 - 60 °C
COLUMN OVEN
Column capacity: 6 x analytical (1 x Tricorn 10/300 GL)
Temperature control range: 20 - 65 °C
OMNISEC REVEAL
Dimensions (W, D, H): 420, 640, 600 mm
Weight: 40 kg
Detector temperature range: 20 - 65 °C
Software: OMNISEC software v10 (or later)
Data acquisition rate: 100 Hz
Patents:
-
US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"
-
US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"
Detector 1
Detector: Light scattering
Measurement principle: RALS 90° angle, LALS 7° angle
Light source: 50 mW, 640 nm laser
Cell volume: 18 µL
Dynamic range: 2500 mV
Baseline noise: <0.1 mV
Baseline drift: <0.2 mV/hr
Detector 2
Detector: Differential refractive index
Measurement principle: Deflection
Cell volume: 12 µL
Dynamic range: ±2.5 x 10-4 RIU
Baseline noise: <10-7 RIU
Baseline drift: <3 x 10-7 RIU/hr
Detector 3
Detector: Viscometer
Measurement principle: 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Transducers: Firmware-based transducer overpressure protection
Detector volume: 17 µL/capillary
Differential pressure baseline noise: 0.3 Pa
Inlet pressure dynamic range: 100 kPa
Inlet pressure baseline noise: 0.01 kPa
Baseline drift: <0.2 kPa/hr
Detector 4
Detector: Diode-array-based UV/Vis spectrometer
Wavelength: 190 - 900 nm
Number of wavelengths: 1024
Wavelength accuracy: <1 nm
Wavelength resolution: 0.6 nm
Cell volume: 7.5 µL
Path length: 10 mm
Baseline noise: 2 x 10-5 AU
Baseline drift: 5 x 10-4 AU/hr
Measurement type 1
Measurement type: Absolute molecular weight
Measurement range: 200 - >107 g/mol
Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle: Light scattering (LALS or RALS)
Measurement type 2
Measurement type: Intrinsic viscosity
Minimum quantifiable mass: 1 µg of 100kDa molecular weight polystyrene in THF
Measurement principle: 4-capillary Wheatstone bridge
Measurement type 3
Measurement type: Concentration
Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF
Measurement principle: Differential refractive index detection
Operating environment
Temperature: 15 - 30 °C
Software
Minimum computer specification:
Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor