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Malvern Panalytical

Malvern Panalytical

OMNISEC The world’s most advanced multi-detector GPC/SEC system Malvern Panalytical's OMNISEC is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system for the measurement of absolute molecular weight, molecular size, intrinsic viscosity, branching and other parameters. It includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.

Key Differences

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OMNISEC is a powerful tool for taking control of the performance, quality and value of your product, whether that’s a synthetic polymer in industry, a natural polymer or polysaccharide used in food or pharmaceutical products, or a protein or antibody used in biopharmaceuticals.

As a research tool, it accelerates research into these molecules by providing absolute information for a wide range of applications making it an ideal tool for universities. It reduces service expense and downtime and increases productivity with easy-to-use intuitive software.

Market leading high sensitive light scattering

  • Measure low molecular weight samples with greater accuracy (down to 200 g/mol)

  • Measure lower sample concentrations (newly synthesized polymers and proteins)

  • Measure low dn/dc samples with greater accuracy and sensitivity 

High sensitivity Refractive index 

  • Greater accuracy and less subjectivity at lower concentrations

  • Measure lower concentraions of highly viscous materials to prevent column overload

Integrated robust differential viscometer 

  • Measure Iv of samples to determine structure and branching 

  • Robust to all GPC solvents and a wide pH range broadening available applications 

Mechanical viscometer bridge autobalance

  • Improved viscometer data quality and accuracy

Fully integrated detector module 

  • Better temperature control and reduced interdetecor volume = better chromatography 

  • Improved separation quality and resolution

All detectors in series 

  • no detectors in a parallel flow path meaning higher sensitivity and better quality results

Work flow orientated software

  • Makes GPC/SEC measurements and analysis easy and intuitive

How it works

In size-exclusion chromatography, molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in the column. Like other HPLC-based techniques, this involves the use of a pump, column and at least one detector to measure the sample as it elutes. An OMNISEC GPC/SEC system includes the following components:


  • Rapid mobile-phase exchange thanks to low volume degasser


  • Rapid mobile-phase exchange thanks to self-priming pump

  • Reduced service requirement thanks to novel continuous back seal flushing mechanism


  • Protect samples from aggregation with autosampler temperature control

  • Use your preferred sample format; vials or 96-well microtiter plates

  • Reduce sample use with zero-waste mode

Column Oven

  • Temperature control of up to 6 analytical columns

Refractive Index

  • Measure the concentration of any solute with excellent sensitivity


  • Measure absorbance at any wavelength from 190-900 nm

  • Assess changes in absorbance spectra

  • Characterize copolymers

Light Scattering

  • Measure absolute molecular weight in a wider range of applications

  • Measure as little as 100 ng of material

  • Measure molecular weights down to 200 Da


  • Better intrinsic viscosity measurements thanks to automated balancing

  • Reduced service cost and time thanks to user-exchangeable capillaries

Technical Specification

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Pump flow rate: 0.005 - 10 mL/min

Flow rate accuracy: ±1%

Pressure range: 0 - 5000 PSI (34.5 MPa)

Pulsation: 0.15% @ 1 mL/min in water

Dimensions (W, D, H): 420, 640, 890 mm

Weight: 62 kg

Software: OMNISEC software v10 (or later)

Data acquisition rate: 100 Hz 


Capacity: Up to 192

Sample container types: HPLC vials or 96-well microtiter plates

Injection volume: 1 - 300 µL

Injection volume accuracy: >99.5%

Injection volume precision:

  • <0.3% RSD in full loop mode

  • <0.5% RSD in partial loop mode

  • <1% RSD in µL pickup mode

Injection overhead volume: 0 µL in µL pickup mode

Syringe volume: 250 µL standard

Temperature control range: 4 - 60 °C


Column capacity: 6 x analytical (1 x Tricorn 10/300 GL)

Temperature control range: 20 - 65 °C


Dimensions (W, D, H): 420, 640, 600 mm

Weight: 40 kg

Detector temperature range: 20 - 65 °C

Software: OMNISEC software v10 (or later)

Data acquisition rate: 100 Hz


  • US20140060162A1 & EP2619543Ba "Modular capillary bridge viscometer"

  • US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"


Detector 1

Detector:  Light scattering

Measurement principle: RALS 90° angle, LALS 7° angle

Light source: 50 mW, 640 nm laser

Cell volume: 18 µL

Dynamic range: 2500 mV

Baseline noise: <0.1 mV

Baseline drift: <0.2 mV/hr


Detector 2

Detector: Differential refractive index

Measurement principle: Deflection

Cell volume: 12 µL

Dynamic range: ±2.5 x 10-4 RIU

Baseline noise: <10-7 RIU

Baseline drift: <3 x 10-7 RIU/hr


Detector 3

Detector: Viscometer

Measurement principle: 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries

Transducers: Firmware-based transducer overpressure protection

Detector volume: 17 µL/capillary

Differential pressure baseline noise: 0.3 Pa

Inlet pressure dynamic range: 100 kPa 

Inlet pressure baseline noise: 0.01 kPa

Baseline drift: <0.2 kPa/hr

Detector 4

Detector: Diode-array-based UV/Vis spectrometer

Wavelength: 190 - 900 nm

Number of wavelengths: 1024

Wavelength accuracy: <1 nm

Wavelength resolution: 0.6 nm

Cell volume: 7.5 µL

Path length: 10 mm

Baseline noise: 2 x 10-5 AU

Baseline drift: 5 x 10-4 AU/hr


Measurement type 1

Measurement type: Absolute molecular weight

Measurement range: 200 - >107 g/mol

Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle: Light scattering (LALS or RALS)


Measurement type 2

Measurement type: Intrinsic viscosity

Minimum quantifiable mass: 1 µg of 100kDa molecular weight polystyrene in THF

Measurement principle: 4-capillary Wheatstone bridge


Measurement type 3

Measurement type: Concentration

Minimum quantifiable mass: 100 ng of 100kDa molecular weight polystyrene in THF

Measurement principle: Differential refractive index detection

Operating environment

Temperature: 15 - 30 °C



Minimum computer specification:

Windows 7 Professional 64 bit, 4th Gen Intel(R) Core I7 Processor (Quad Core HT, 3.4 GHz Turbo, w/ HD Graphics), 8 GB 1600 MHz DDR3 memory, 500 GB 3.5 inch SATA (7200 RPM) HDD, Full-HD monitor



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