Rapid, automated, component-specific particle characterization in a single, integrated platform Morphologi 4-ID delivers detailed component-specific morphological descriptions of particulate blends through Morphologically-Directed Raman Spectroscopy (MDRS). This combines automated particle imaging with Raman spectroscopy in a single, integrated platform, providing the power to unlock complex particle characterization problems.
The Morphologi 4-ID offers an exclusive capability, combining all the benefits of automated static imaging delivered by the Morphologi 4 with chemical identification of individual particles by Raman spectroscopy in a single measurement.
Patented MDRS capability delivers representative component-specific particle size and shape data, delivering full characterization of your sample
All the functionality of the Morphologi 4 combined with a dedicated Raman platform for both physical and chemical particle characterization in a single, automated measurement
Automatically measures Raman spectra for hundreds or thousands of particles, saving valuable analyst time
Intuitive software ensures suitability for experienced and non-experienced spectroscopists alike
Easy correlation of morphological properties with chemical information provides the most comprehensive understanding of your sample
21 CFR Part 11 software option ensures regulatory compliance
Adaptable workflows enable methods to be tailored to specific user or application requirements. Select particles for chemical analysis:
manually, from particle images
morphologically-directed, based on user-specified classifications (MDRS)
automatically and objectively by the software
Ability to export spectra in industry-standard format supports unknown component identification using 3rd party spectral libraries
Tight control of laser power and acquisition time enables measurement optimization for a wide range of materials, from weak Raman scatterers to those that are thermally sensitive
The Morphologi 4-ID measurement procedure can be split into five sections:
Spatial separation of individual particles and agglomerates is critical to robust results. The integrated dry powder disperser makes preparing dry powder samples easy and reproducible. The applied dispersion energy can be precisely controlled, enabling the measurement process to be optimized for a range of material types. Accessories that fit directly in to the Morphologi 4-ID’s automated stage are available for preparing suspended or filtered samples.
The instrument captures images of individual particles by scanning the sample underneath the microscope optics. The Morphologi 4-ID can illuminate the sample from below or above, whilst accurately controlling the light levels.
Use of either the automated ‘Sharp Edge’ segmentation analysis or the manually-controlled thresholding enables the detection of particles and the calculation of a range of morphological parameters for each.
Components and particles of interest are targeted for Raman analysis. Particles can be selectively targeted based on their morphology, or particles representative of the whole sample can be targeted objectively by the Morphologi software.
Advanced graphing and data classification options in the software ensure that extracting the relevant data from the measurement is straightforward, via an intuitive visual interface. Correlation scores to a reference library are calculated for each Raman spectrum acquired, enabling particles to be classified based on their chemistry. The morphological data associated with the particles in each chemical class is used to generate size and shape distributions, delivering component-specific morphological information. Individually-stored grayscale images for each particle are linked to their Raman spectra and provide qualitative verification of the quantitative results.
Technology: Static automated imaging
Particle size: 0.5 μm – 1300 μm (upper limit may be extended for some applications*)
Particle properties measured: Size, shape, transparency, count, location
Particle size parameters: Circle equivalent (CE) diameter, length, width, perimeter, area, maximum distance, sphere equivalent (SE) volume, fiber total length, fiber width
Particle shape parameters: Aspect ratio, circularity, convexity, elongation, high sensitivity (HS) circularity, solidity, fiber elongation, fiber straightness
Particle transparency parameters: Intensity mean, intensity standard deviation
Integrated Sample Dispersion Unit: For fully automated dispersion and measurement of dry powders. Manual or SOP control of dispersion pressure, injection time and settling time
Illumination: White light LED: brightfield, diascopic and episcopic; darkfield, episcopic
Detector: 18 MP; 4912 x 3684 pixel color CMOS array; pixel size 1.25 μm x 1.25 μm
Optical system: Nikon CFI 60 brightfield / darkfield system
Lens (and particle size range):
2.5x: 8.5 µm – 1300 µm (nominal)
5x: 4.5 µm – 520 µm (nominal)
10x: 2.5 µm – 260 µm (nominal)
20x: 1.5 µm – 130 µm (nominal)
50x: 0.5 µm – 50 µm (nominal)
Size range: 1 μm - 1300 μm*
Spectral performance: 150 cm-1 to 2800 cm-1
6 cm-1 resolution measured on an atomic line source. Average of < 8 cm-1 across the range.
Wavelength: 785 nm
Power output from spectrometer: < 100 mW
Power output at sample: > 45 mW at max power
Laser spot size: 2 µm at 50x magnification
Laser safety: Class 1
Chemical identification method: Raman spectral correlation
Weight and dimensions:
Dimensions (W, D, H): 810 mm (W) x 520 mm (D) x 685 mm (H)
with carry handles: 1100mm (W) x 520mm (D) x 685mm (H)
Weight: 80 kg (88 kg including carry handles)
Power: 100-240 V ac 50/60 Hz (<100W load)
Notes: * Sample and substrate dependent