AF2000 MultiFlow FFF - Universal Separator

BY
Postnova Analytics
Postnova Analytics
Description

The most Advanced Flow FFF Platform for Separation of Proteins, Macromolecules and Nanoparticles!
Professional Modular Platform
The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors. The AF2000 Series incorporates the combined solid know-how and the technologies from three decades of leadership in FFF. Due to its unique design, the AF2000 MultiFlow platform offers more flexibility, higher robustness and better performance than any system before. The technology sets a new standard in the area of Flow FFF and marks a real break-through in FFF science and therefore naturally has become the leading Flow FFF solution for many scientists around the world.
Universal Flexible Separator
The AF2000 Series platform is based on the Flow FFF principle, using a crossflow field as driving force for the separation. The samples, which are affected by this field, are separated by their Dynamic Diffusion on the basis of the molar mass or particle size. The AF2000 can be run with different eluents, at various temperature conditions and is able to employ various channel dimensions and principles. The same system can be used for running planar Asymmetric Flow FFF (AF4) channels or circular HollowFiber Flow FFF (HF5) channels. Therefore, the AF2000 MultiFlow can be easily used for separation of a broad range of different samples from the area of Biopharmaceuticals, Food-Agro-Cosmetics, Environmental, Chemicals and Nanotechnology. The MultiFlow FFF is ideal for the characterization of proteins, antibodies, aggregates, vaccines, liposomes, nanoparticles and natural or synthetic macromolecules. Because of its great flexibility, the AF2000 MultiFlow FFF can be employed as a Universal Separator for many kind of analyte systems and is a secure investment also for new upcoming samples and future project demands.
AF2000 AT - Standard Ambient Temp Flow FFF
The AF2000 AT is a non-temperature controlled economical Flow FFF which is ideal for the use in general level research in universities, companies and public scientific centers. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily. The AF2000 MT is a temperature controlled advanced Flow FFF system which is ideal for the use in higher level research in university and public research institutions as well as professional research and commercial-industrial applications in the area of product development, manufacturing and quality control. It is also available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily. Temperature control is not only available for the separation channel but also for the entire system, including the transfer capillaries between the various modules and detectors.
AF2000 HT - Top-Level High Temp Flow FFF
The AF2000 HT is a special temperature controlled top-level Flow FFF system which is ideal for the use in research, development and quality control of polyolefins and high temperature macromolecules in universities, public research institutions and industry. It is available as stainless steel version and can be run with organic solvents from ambient temperature up to high temperature conditions. Special planar Asymmetric Flow FFF (AF4) channels made from stainless steel and ceramics are exclusively employed for this conditions. Infrared (IR), Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Viscometry (Visco) can be used as detection principles .
Advanced Flow FFF Detection
In order to enable the user to fully access this wide application range, the AF2000 MultiFlow is available in different unique versions. Various add-on modules, extra options and special FFF detectors are available, such as Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). All Postnova FFF modules and detectors have been especially developed for FFF and are perfectly optimized not only for the use with the AF2000 MultiFlow FFF but also for the other Postnova FFF versions. Additionally, the AF2000 Series has been designed for easy interfacing with existing high-end detection technologies from leading instrument suppliers so that the user can ideally integrate the Postnova FFF technology into the existing laboratory infrastructure in order to maximize efficiency and investment.

Key Differences
Broad Separation Range

The AF2000 platform can separate molecules and particles from 1 nm up into the µm range. This can be achieved using the same separation channel by changing the separation programs via the powerful NovaFFF software. This also means that mixtures of molecules and particles can be separated in the same experiment, for example proteins and nanoparticles in drug-delivery development.

Gentle Separation Conditions

Because of the open channel and the absence of any stationary phase, the separation is performed under the absence of shear forces and stress to the particles or macromolecule. Typical chromatography problems, such as filtering/adsorption effects and shear induced degradation can be avoided.

FOCUS Technology

The AF2000 platform incorporates Postnova’s FOCUS technology to ensure a continuous and stable detector flow at all times. This provides a more stable and reproducible detector signals and is an absolute necessity to be able to connect flow sensitive devices such as refractive index (RI) or viscometer detectors. Additionally, the technology provides higher sample recovery, resolution and sensitivity. Another striking advantage of the Focus technology is the channel outlet which can be directly connected to the detector without any valves or T-pieces included. This ensures short transfer lines, low dead volumes and minimized band broadening.

Cross Flow System

Postnova uses advanced technology for generating the cross flow inside the FFF channel based on a dual syringe pump that is controlled in a unique way to minimise any pulsation. The pump is completely inert and can easily produce constant and absolute flows ranging from 0.005 to 10 mL/min for different solvents. By using this volumetric based syringe pump technology, Postnova can ensure the cross flow rate is reproducible, stable and independent of the solvent composition and temperature.

Flexibility & Interfaceability

The AF2000 series was developed as a modular system to give the maximum flexibility for system configuration and upgradeability. In addition, the open architecture of the system allows the integration of many components including detectors from 3rd party companies such as DLS and ICP-MS.

Technical Specification
Temperature Range:
  • Room Temperature

Principle Measurement Range:
  • Particles: 1 nm to 100 µm

  • Polymers: 500 Da to 1012 Da

Channel Dimensions:
  • Volume: 0,5 -2,5 mL

  • Size: 335 x 60 x 40 mm

  • Thickness: 350 µm (other sizes available on request)

Carrier Liquids:
  • Aqueous: any aqueous liquid, pH from 2 - 11 (depending on acid and conditions, please inquire), ionic strength from DI water to saline

  • Organic: THF, MeOH, etc

Membranes:
  • reg. cellulose, PC, PES

  • Molar Mass: 0,3; 1; 2; 4; 5; 10; 30; 100 kDa

Detectors:
  • UV

  • RI

  • Laser Light Scattering

  • Viscometry

  • Fluorescence and more

  • Multiple Detectors in line

Channel Flow Range:
  • 0 - 10 mL/ min

Cross Flow Range:
  • 0 - 8,5 mL /min

Pressure Limit:
  • 0 - 35 bar

Power Requirements:
  • 110 V or 240 V, 50/ 60 Hz; 2,5 A

PC Requirements:
  • Windows OS, min.64 MB RAM, 2 RS232 ports

Software System:
  • NovaFFF AF2000 Control

  • NovaFFF Analysis

Maintenance Parts:
  • Consumables

  • Pistons and piston seals

  • Inline filters

  • Membranes

Injection Volume:
  • 1 - 1000 µL

  • Optional > 1000 µL

Injected Sample Mass:
  • Via manual injection valve

  • Via PN5300 autosampler

Downloads
AF2000 MultiFlow FFF - Universal Separator