AF2000 MultiFlow FFF - Universal Separator


Postnova Analytics

Postnova Analytics

The most Advanced Flow FFF Platform for Separation of Proteins, Macromolecules and Nanoparticles!
Professional Modular Platform
The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors. The AF2000 Series incorporates the combined solid know-how and the technologies from three decades of leadership in FFF. Due to its unique design, the AF2000 MultiFlow platform offers more flexibility, higher robustness and better performance than any system before. The technology sets a new standard in the area of Flow FFF and marks a real break-through in FFF science and therefore naturally has become the leading Flow FFF solution for many scientists around the world.
Universal Flexible Separator.

Key Differences

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Broad Separation Range

The AF2000 platform can separate molecules and particles from 1 nm up into the µm range. This can be achieved using the same separation channel by changing the separation programs via the powerful NovaFFF software. This also means that mixtures of molecules and particles can be separated in the same experiment, for example proteins and nanoparticles in drug-delivery development.

Gentle Separation Conditions

Because of the open channel and the absence of any stationary phase, the separation is performed under the absence of shear forces and stress to the particles or macromolecule. Typical chromatography problems, such as filtering/adsorption effects and shear induced degradation can be avoided.

FOCUS Technology

The AF2000 platform incorporates Postnova’s FOCUS technology to ensure a continuous and stable detector flow at all times. This provides a more stable and reproducible detector signals and is an absolute necessity to be able to connect flow sensitive devices such as refractive index (RI) or viscometer detectors. Additionally, the technology provides higher sample recovery, resolution and sensitivity. Another striking advantage of the Focus technology is the channel outlet which can be directly connected to the detector without any valves or T-pieces included. This ensures short transfer lines, low dead volumes and minimized band broadening.

Cross Flow System

Postnova uses advanced technology for generating the cross flow inside the FFF channel based on a dual syringe pump that is controlled in a unique way to minimise any pulsation. The pump is completely inert and can easily produce constant and absolute flows ranging from 0.005 to 10 mL/min for different solvents. By using this volumetric based syringe pump technology, Postnova can ensure the cross flow rate is reproducible, stable and independent of the solvent composition and temperature.

Flexibility & Interfaceability

The AF2000 series was developed as a modular system to give the maximum flexibility for system configuration and upgradeability. In addition, the open architecture of the system allows the integration of many components including detectors from 3rd party companies such as DLS and ICP-MS.

Technical Specification

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Temperature Range:
  • Room Temperature

Principle Measurement Range:
  • Particles: 1 nm to 100 µm

  • Polymers: 500 Da to 1012 Da

Channel Dimensions:
  • Volume: 0,5 -2,5 mL

  • Size: 335 x 60 x 40 mm

  • Thickness: 350 µm (other sizes available on request)

Carrier Liquids:
  • Aqueous: any aqueous liquid, pH from 2 - 11 (depending on acid and conditions, please inquire), ionic strength from DI water to saline

  • Organic: THF, MeOH, etc

  • reg. cellulose, PC, PES

  • Molar Mass: 0,3; 1; 2; 4; 5; 10; 30; 100 kDa

  • UV

  • RI

  • Laser Light Scattering

  • Viscometry

  • Fluorescence and more

  • Multiple Detectors in line

Channel Flow Range:
  • 0 - 10 mL/ min

Cross Flow Range:
  • 0 - 8,5 mL /min

Pressure Limit:
  • 0 - 35 bar

Power Requirements:
  • 110 V or 240 V, 50/ 60 Hz; 2,5 A

PC Requirements:
  • Windows OS, min.64 MB RAM, 2 RS232 ports

Software System:
  • NovaFFF AF2000 Control

  • NovaFFF Analysis

Maintenance Parts:
  • Consumables

  • Pistons and piston seals

  • Inline filters

  • Membranes

Injection Volume:
  • 1 - 1000 µL

  • Optional > 1000 µL

Injected Sample Mass:
  • Via manual injection valve

  • Via PN5300 autosampler

AF2000 MultiFlow FFF - Universal Separator


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